Journal of Paramedical Sciences & Rehabilitation

Journal of Paramedical Sciences & Rehabilitation

Effect of Photodynamic Therapy with 808 Nm Diode Laser And Different ‎Concentrations of Indocyanine Green on Promastigotes of Leishmania Major

Document Type : Original Article

Authors
Sh Jalili 1
M Hoseini 2
H.R Sadeghi 2
S.A Mousavi Nejad 3
1 Institute of Police Equipment and Technologies, Policing Sciences and Social Studies Research Institute, Tehran, Iran
2 Department of Radiology Technology, School of Paramedical Sciences, Torbat Heydarieh University of Medical Sciences, Torbat Heydarieh, Iran
3 Research Center for Life and; Health Sciences; Biotechnology of the Police, Directorate of Health, Rescue; Treatment, Police Headquarter, Tehran, Iran
10.22038/jpsr.2024.78799.2610
Abstract
Purpose:
Leishmaniasis is a common infectious agent of humans and animals that threatens more than 350 million people in 100 countries including Iran, and still has no effective and low-complication treatment. Photodynamic therapy (PDT) is a non-invasive and repeatable method for removing leishmaniasis lesions. The aim of this study was to investigate the effect of photodynamic therapy with the near-infrared laser in the presence of indocyanine green (ICG) on leishmaniasis.
Methods:
In vitro study was carried out after amplification of parasites extracted from the spleen of infected mice in the presence of 6 different concentrations of ICG and exposure with the 808 nm laser. To avoid the photothermal effect, the output intensity was limited to 1 W/cm2. Each study included three controls: untreated parasites, ICG-treated parasites and laser beam-exposed parasites. Finally, the effectiveness of the treatments was evaluated by the MTS/PMS method. 
Results:
There was no significant difference in survival in all groups containing ICG compared to the control group (P>0.05), but these groups showed a significant reduction in survival compared to the control group after exposure (P<0.05). Also, in the group without ICG, just laser exposure did not decrease cell survival compared to the control group (P>0.05).
Conclusion:
The findings of this study showed that photodynamic therapy in the presence of ICG could be a new, safe and low-cost option against L. major.
Keywords
Leishmaniasis
Photodynamic therapy
Indocyanine green
Near-infrared laser
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Journal of Paramedical Sciences & Rehabilitation
Volume 13, Issue 2
Summer 2024
Pages 7-16