Identification of Biofilm Formation Genes in Candida albicans and Evaluation of Iron Nanoparticles on the Expression of als gene by Real Time-PCR

Document Type : Original Article

Authors

Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran.

Abstract

Purpose:
Approximately three-fourths of women experience an episode of vaginal candidiasis. One of the virulence factors, is the ability of attachment of C. albicans to the host tissue through factors such as sticky molecules and proteins that accelerate this connection. The aim of this study was to investigate the frequency of biofilm formation genes in C. albicans strains isolated from vaginosis and to compare the expression of als gene in strains treated with iron nanoparticles in the in vitro using real time -PCR.
Methods:
In this descriptive cross-sectional study conducted in Shariati Hospital during a 6-months period, 100 isolates Candidafrom vaginal specimens were evaluated. After identification of C. albicans strains using standard biochemical and microbiological tests, genomic DNA extraction was performed using the CinnaGen Company Kit. Multiplex-PCR assays were performed to detect als, hwp1 and hyr genes. Also, to investigate the effect of iron nanoparticle on expression of als gene, RNA, all isolates containing this gene were extracted using Cinnapure kit. After cDNA synthesis, real time PCR test was performed.
Results:
Molecular analysis results showed that from the total of 60 isolates of C. albicans, 34 isolates (56.66%) had the hwp gene, 46 isolates (76.66%) had Als gene and 33 isolates (55%) had Hyr gene. The results of the asl melting curve in clinical strains of C. albicans and treated with iron nanoparticles showed that the main peak for this gene occurs at 85.34 ° C.
Conclusions:
Als expression in the treated isolates was lower than untreated, which indicates the positive role of the treatment in reducing Als gene expression in these isolates.

Keywords

References

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Journal of Paramedical Sciences & Rehabilitation
Volume 8, Issue 3
October 2019
Pages 25-33

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