The Effect of Eight Weeks of Intermittent Rehabilitation Training with Quercetin ‎Nanoliposome on the Apoptotic Indices of P38 and MK2 in Male Rats with Isoproterenol ‎Induced Myocardial Infarction Model

Document Type : Original Article

Authors

1 Department of Sports Physiology, Faculty of Sports Sciences, Isfahan (Khorasgan) Branch, ‎Islamic Azad University, Isfahan, Iran

2 Department of Sports Physiology, Faculty of Sports Sciences, Isfahan (Khorasgan) Branch, ‎Islamic Azad University, Isfahan, Iran‎

3 Department of Sport Physiology, Faculty of Sport Sciences, University of Isfahan, Isfahan, ‎Iran

Abstract

Purpose:
Quercetin is effective in the rehabilitation and treatment of apoptosis caused by cardiac ischemia, however, due to its low solubility in water, it is not suitable for clinical application, which can be improved by turning it into nanoparticles. The present study was designed to determine the effect of liposome nanoparticles with quercetin on apoptotic indices P38 and MK2 and its mechanisms in the rehabilitation of ischemic heart damage in rats.
Methods:
30 rats weighing 250±20 grams (aged 6-8 weeks) were randomly divided into five groups (healthy, sick, sick+supplement, sick+exercise and sick+exercise+supplement). Myocardial infarction was performed with isopronalin as a subcutaneous injection. Supplemental gavage was performed after training (8 sessions of aerobic training including: 5 days of training per week, which includes 7 intervals) and it was performed with a dose of 10 mg/kg of body weight of mice in the supplement receiving groups for eight weeks in the supplement receiving groups  48 hours after the last training session. The expression level of P38 and MK2 genes was measured by Real-Time PCR method. Data analysis was done with one-way analysis of variance, ANOVA, and Tukey's test at a significance level of p<0.05.
Results:
The expression of the MK2 gene in the heart tissue in the exercise and supplement groups decreased significantly, and this decrease was greater in the exercise and supplement consumption group (P=0.0001). However, in the expression of the P38 gene in the exercise and supplement groups, no significant decrease was found (P=0.0516).
Conclusion:
The consumption of nanoliposomal quercetin along with intermittent exercises significantly increased the effect of quercetin in reducing the expression of MK2 gene, which prevents the activation of the P38-MK2 complex, therefore it has a protective effect on cardiac tissue after ischemic damage.

Keywords

References

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Journal of Paramedical Sciences & Rehabilitation
Volume 13, Issue 1
April 2024
Pages 42-55

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