Effect of Photodynamic Therapy with 808 Nm Diode Laser And Different ‎Concentrations of Indocyanine Green on Promastigotes of Leishmania Major

Document Type : Original Article

Authors

1 Institute of Police Equipment and Technologies, Policing Sciences and Social Studies Research Institute, Tehran, Iran

2 Department of Radiology Technology, School of Paramedical Sciences, Torbat Heydarieh University of Medical Sciences, Torbat Heydarieh, Iran

3 Research Center for Life and; Health Sciences; Biotechnology of the Police, Directorate of Health, Rescue; Treatment, Police Headquarter, Tehran, Iran

Abstract

Purpose:
Leishmaniasis is a common infectious agent of humans and animals that threatens more than 350 million people in 100 countries including Iran, and still has no effective and low-complication treatment. Photodynamic therapy (PDT) is a non-invasive and repeatable method for removing leishmaniasis lesions. The aim of this study was to investigate the effect of photodynamic therapy with the near-infrared laser in the presence of indocyanine green (ICG) on leishmaniasis.
Methods:
In vitro study was carried out after amplification of parasites extracted from the spleen of infected mice in the presence of 6 different concentrations of ICG and exposure with the 808 nm laser. To avoid the photothermal effect, the output intensity was limited to 1 W/cm2. Each study included three controls: untreated parasites, ICG-treated parasites and laser beam-exposed parasites. Finally, the effectiveness of the treatments was evaluated by the MTS/PMS method. 
Results:
There was no significant difference in survival in all groups containing ICG compared to the control group (P>0.05), but these groups showed a significant reduction in survival compared to the control group after exposure (P<0.05). Also, in the group without ICG, just laser exposure did not decrease cell survival compared to the control group (P>0.05).
Conclusion:
The findings of this study showed that photodynamic therapy in the presence of ICG could be a new, safe and low-cost option against L. major.

Keywords

References

  1. Bilgic-Temel A, Murrell DF, Uzun S. Cutaneous leishmaniasis: A neglected disfiguring disease for women. Int J Womens Dermatol 2019; 5(3): 158-165.
  2. Reithinger R, Dujardin J-C, Louzir H, Pirmez C, et al. Cutaneous leishmaniasis. Lancet Infect Dis 2007; 7(9): 581-596.
  3. Severino P, Santana W, Lisboa ES, Santos VLSd, et al. Cutaneous/Mucocutaneous Leishmaniasis Treatment for Wound Healing: Classical versus New Treatment Approaches. Microbiol Res 2022; 13(4):-836-852.
  4. Azim M, Khan SA, Ullah S, Ullah S, Anjum SI. Therapeutic advances in the topical treatment of cutaneous leishmaniasis: A review. PLoS Negl Trop Dis 3021; 15(3): e0009099.
  5. Ozlem-Caliskan S, Ertabaklar H, Bilgin MD, Ertug S. Evaluation of photodynamic therapy against Leishmania tropica promastigotes using different photosensitizers. Photodermatol Photoimmunol Photomed 2022; 38(4):354-364.
  6. Kim H, Lee YR, Jeong H, Lee J, et al. Photodynamic and photothermal therapies for bacterial infection treatment. Smart Molecules 2023; 1(1): e20220010
  7. Ozlem-Caliskan S, Ilikci-Sagkan R, Karakas H, Sever S, et al. Efficacy of malachite green mediated photodynamic therapy on treatment of Cutaneous Leishmaniasis: In vitro study. Photodiagnosis Photodyn Ther 2022; 40: 103111.
  8. Linares IAP, Uría MS, Graminha MAS, Iglesias BA, et al. Antileishmanial activity of tetra-cationic porphyrins with peripheral Pt(II) and Pd(II) complexes mediated by photodynamic therapy. Photodiagnosis Photodyn Ther 2023; 42: 103641.
  9. Pinto JG, Fontana LC, de Oliveira MA, Kurachi C, et al. In vitro evaluation of photodynamic therapy using curcumin on Leishmania major and Leishmania braziliensis. Lasers Med Sci 2016; 31(5): 883-890.
  10. de Oliveira de Siqueira LB, da Silva Cardoso V, Rodrigues IA, Vazquez-Villa AL, et al. Development and evaluation of zinc phthalocyanine nanoemulsions for use in photodynamic therapy for Leishmania spp. Nanotechnology 2017; 28(6): 065101.
  11. Navasconi TR, Dos Reis VN, Freitas CF, Pereira PCS, et al. Photodynamic Therapy with Bengal Rose and Derivatives Against Leishmania amazonensis. J Lasers Med Sci 2017; 8(1): 46-50
  12. Escobar P, Vera AM, Neira LF, Velásquez AO, Carreno H. Photodynamic therapy using ultradeformable liposomes loaded with chlorine aluminum phthalocyanine against L. (V.) braziliensis experimental models. Exp Parasitol 2018; 194: 45-52
  13. Marcolino LMC, Pereira AHC, Pinto JG, Mamone LA, Strixino JF . Cellular and Metabolic Changes after Photodynamic Therapy in Leishmania Promastigotes. Photodiagnosis Photodyn Ther 2021; 35: 102403.
  14. Najm M, Pourhajibagher M, Badirzadeh A, Razmjou E, et al. Photodynamic Therapy Using Toluidine Blue O (TBO) Dye as a Photosensitizer against Leishmania major. Iran J Public Health 2021; 50(10): 2111-2120.
  15. Vital-Fujii DG, Baptista MS. Progress in the photodynamic therapy treatment of Leishmaniasis. Braz J Med Biol Res 2021; 54(12): e11570.
  16. Ghorbani F, Attaran-Kakhki N, Sazgarnia A. The synergistic effect of photodynamic therapy and photothermal therapy in the presence of gold-gold sulfide nanoshells conjugated Indocyanine green on HeLa cells. Photodiagnosis Photodyn Ther 2017; 17: 48-55.
  17. El-Daly SM, Gamal-Eldeen AM, Abo-Zeid MA, Borai IH, et al. Photodynamic therapeutic activity of indocyanine green entrapped in polymeric nanoparticles. Photodiagnosis Photodyn Ther 2013; 10(2): 173-185.
  18. Shirata C, Kaneko J, Inagaki Y, Kokudo T, et al. Near-infrared photothermal/photodynamic therapy with indocyanine green induces apoptosis of hepatocellular carcinoma cells through oxidative stress. Sci Rep 2017; 7(1): 13958.
  19. Ting C-W, Chou Y-H, Huang S-Y, Chiang W-H. Indocyanine green-carrying polymeric nanoparticles with acid-triggered detachable PEG coating and drug release for boosting cancer photothermal therapy. Colloids Surf B Biointerfaces 2021; 208: 112048.
  20. Yan L, Qiu L. Indocyanine green targeted micelles with improved stability for near-infrared image-guided photothermal tumor therapy. Nanomedicine (Lond) 2015; 10(3): 361-373.
  21. 21.Ara ES, Noghreiyan AV, Sazgarnia A. Evaluation of photodynamic effect of Indocyanine green (ICG) on the colon and glioblastoma cancer cell lines pretreated by cold atmospheric plasma. Photodiagnosis Photodyn Ther 2021; 35: 102408.
  22. Bilici K, Atac N, Muti A, Baylam I, et al, Sennaroglu A, et al. Broad spectrum antibacterial photodynamic and photothermal therapy achieved with indocyanine green loaded SPIONs under near infrared irradiation. Biomater Sci 2020; 8(16): 4616-4625.
  23. Mohebali M, Rezayat M, Gilani K, Sarkar S, et al. Nanosilver in the treatment of localized cutaneous leishmaniasis caused by Leishmania major (MRHO/IR/75/ER): an in vitro and in vivo study. DARU J Pharm Sci 2015; 17(4):285-289
  24. Sazgarnia A, Zabolinejad N, Layegh P, Rajabi O, et al. Antileishmanial Activity of Liposomal Clarithromycin against Leishmania Major Promastigotes. Iran J Basic Med Sci 2012; 15(6): 1210-1214.
  25. Dolat E, Rajabi O, Salarabadi SS, Yadegari‐Dehkordi S, Sazgarnia A. Silver nanoparticles and electroporation: their combinational effect on Leishmania major. Bioelectromagnetics 2015; 36(8): 586-596.
  26. 26  Kim K, Park H, Lim KM. Phototoxicity: Its Mechanism and Animal Alternative Test Methods. Toxicol Res. 2015;31:97-104.
  27. Hao Y, Chung CK, Gu Z, Schomann T, et al. Combinatorial therapeutic approaches of photodynamic therapy and immune checkpoint blockade for colon cancer treatment. Mol Biomed 2022; 3(1):26.
  28. Huang YY, Chen AC, Carroll JD, Hamblin MR. Biphasic dose response in low level light therapy. Dose Response 2009; 7(4): 358-383.
  29. Onda N, Kimura M, Yoshida T, Shibutani M. Preferential tumor cellular uptake and retention of indocyanine green for in vivo tumor imaging. Int J Cancer 2016; 139(3): 673-682.
  30. Song D, Lindoso JA, Oyafuso LK, Kanashiro EH, et al. Photodynamic therapy using methylene blue to treat cutaneous leishmaniasis. Photomed Laser Surg 2011; 29(10): 711-715.
  31. Akilov OE, Kosaka S, O'Riordan K, Hasan T. Photodynamic therapy for cutaneous leishmaniasis: the effectiveness of topical phenothiaziniums in parasite eradication and Th1 immune response stimulation. Photochem Photobiol Sci 2007; 6(10): 1067-1075.
  32. Aureliano DP, Lindoso JAL, de Castro Soares SR, Takakura CFH, et al. Cell death mechanisms in Leishmania amazonensis triggered by methylene blue-mediated antiparasitic photodynamic therapy. Photodiagnosis Photodyn Ther 2018; 23: 1-8.
  33. Kirchherr A-K, Briel A, Mäder K. Stabilization of Indocyanine Green by Encapsulation within Micellar Systems. Mol Pharm 2009; 6(2): 480-491.
Journal of Paramedical Sciences & Rehabilitation
Volume 13, Issue 2
July 2024
Pages 7-16

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