تداخلات سلولی و ملکولی اپی‌تلیوم قرنیه با فلوروسئین سدیم

نوع مقاله: مقاله مروری

نویسندگان

1 دانشجوی دکترای تخصصی اپتومتری، گروه آموزشی اپتومتری، دانشکده علوم پیراپزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران - مرکز تحقیقات عیوب انکساری چشم، دانشکده علوم پیراپزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران

2 کارشناس ارشد اپتومتری، مرکز تحقیقات چشم، بیمارستان چشم پزشکی فارابی، دانشگاه علوم پزشکی تهران، تهران، ایران

چکیده

فلوروسئین سدیم (فلوروسئین) در رنگ‌آمیزی اپی‌تلیوم قرنیه به منظور ارزیابی اینتگریتی سطح چشم بطور گسترده‌ای به کار گرفته می‌شود. فلوروسانس سطح چشم که بطور بالینی مشاهده می‌شود بوسیله فاکتورهای متعددی از قبیل غلظت فلوروسئین، ضخامت لایه فلوروسئین، طول موج منبع نوری تحریک کننده، اسیدیته اشک و سایر عوامل تحت تأثیر قرار می‌گیرد. علیرغم استفاده گسترده فلوروسئین به منظور بررسی سطح چشم، شناخت و تفسیر بالینی فلوروسانس سطح قرنیه و عوامل اصلی مکانیسم ایجاد کننده این پدیده، بطور کامل در نظر گرفته نمی‌شوند. علاوه بر این، فهم بهتر رنگ‌آمیزی فلوروسئینی قرنیه و تظاهرات بالینی آن، نیازمند شناخت خواص فیزیکی-شیمیایی پایه و ملکولی رنگ فلوروسئین و میکرو آناتومی و فیزیولوژی قرنیه میباشد. در این مقاله مروری، مقالات منتشر شده در PubMed بین سالهای 1970 تا 2015 مورد ارزیابی و نقد قرار گرفته، شواهد علمی معتبر با توجه به معیارهای ورود و خروج، جمع‌آوری، بررسی و نتیجه‌گیری شدند. هدف از این مطالعه، مروری بر تداخلات اساسی بین سلولهای سطح قرنیه و ملکولهای فلوروسئین در رنگ‌آمیزی سطحی قرنیه‌ای می‌باشد.  

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